Classical and Basal Cell Culture Media
Optimize Technologies Medium Seal Forming/Insertion Tool
Installing a piston seal can be a tricky undertaking. A relatively small seal must fit into the seal cavity in such a way to ensure the seal is inserted evenly without distortion or damage. The Optimize Seal Forming/Insertion Tool Kit is specifically designed for easy and successful installation. One end has a beveled cavity for seal forming while the other end is an insertion support for perfect seal installation. Each kit contains instructions for maximizing seal life and a Solvent Compatibility Guide to aid in seal material selection.
STEMCELL Technologies PneumaCult™-ALI Medium with 6.5 mm Transwell Inserts, Size: 1 Kit
Medium and Transwell inserts for human airway epithelial cells cultured at the air-liquid interface
STEMCELL Technologies PneumaCult™-ALI Medium
PneumaCult™-ALI Medium (Catalog #05001) is a serum- and BPE-free medium for the culture of human airway epithelial cells at the air-liquid interface (ALI). Airway epithelial cells cultured in PneumaCult™-ALI Medium undergo extensive mucociliary differentiation to form a pseudostratified epithelium that exhibits morphological and functional characteristics similar to those of the human airway in vivo. PneumaCult™-ALI Medium is also available in a kit that includes 12 mm Transwell inserts (Catalog #05021) or 6.5 mm Transwell inserts (Catalog #05022).Together, PneumaCult™-ALI Medium and PneumaCult™-Ex Plus Medium (Catalog #05040) constitute a fully integrated BPE-free culture system for in vitro human airway modeling that is also compatible with primary human nasal epithelial cells. This robust and defined system is a valuable tool for basic respiratory research, toxicity studies, and drug development.
Sigma Aldrich Fine Chemicals Biosciences Human Bronchial Epithelial
HBEpC provide an excellent model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, inflammation, pulmonary diseases and smoking. When grown on inserts and provided with the liquid/air interface, HBEpC can differentiate into a pseudostriated epithelium and serve as a more physiological 3D tissue model for in vitro studies.