
DMEM-F-12 Cell Culture Media
DMEM/F-12 cell culture media is a serum-free formulation that combines Dulbecco's modified Eagle medium (DMEM) and Ham's F-12 nutrient mixture in a 1:1 ratio. It is designed to provide a suitable environment for the growth and maintenance of various cell types in laboratory settings. This medium is enriched with essential nutrients, including insulin, transferrin, and growth factors, supporting the optimal proliferation and survival of cells1. DMEM/F-12 cell culture media has been widely used for culturing primary mammalian cells as well as various normal and transformed cell lines. Its versatility and ability to support a broad range of cell types make it a valuable tool in cellular research and biotechnological applications.
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Gibco™ DMEM/F-12, GlutaMAX™ supplement
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DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.


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Sterilization Method | Sterile-filtered |
---|---|
With Additives | High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate |
Product Type | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
Form | Liquid |
Without Additives | No HEPES |
Classification | Animal Origin-free |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Gibco™ BenchStable™ DMEM/F12
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Gibco DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells.

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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Cell Line | MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts |
Content And Storage | Store at room temperature. Protect from light. |
Form | Liquid |
Without Additives | No HEPES |
Manufacturing Quality | cGMP-compliant under the ISO 9001: 2015 standard |
Green Features | Energy efficient, Fewer resources used, Sustainable packaging |
Concentration | 1 X |
Sterility | Sterile-filtered |
With Additives | High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate |
Product Type | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
Classification | Animal Origin-free |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | BenchStable™ |
Serum Level | Standard Serum Supplementation |
Gibco™ KnockOut™ DMEM/F-12
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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KnockOut™ DMEM/F-12


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Gibco™ Advanced DMEM/F-12
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Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.


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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Cell Line | MRC-5, SP2, Vero, WI-38 and Jurkat |
Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
Form | Liquid |
Without Additives | No Glutamine,No HEPES |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Sterilization Method | Sterile-filtered |
With Additives | High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA) |
Product Type | DMEM (Dulbecco's Modified Eagle Medium)/F-12 |
Classification | Animal Origin |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | Gibco™ |
Serum Level | Reduced Serum |
Gibco™ CTS™ KnockOut™ DMEM/F-12
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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CTS KnockOut DMEM/F-12 is a serum free, low osmolality medium without L-glutamine or HEPES buffer that is optimized for the growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC).

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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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