Neuromics, Inc BioLock Biotin Blocking Solution, 50 ml

Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media.Thus, if proteins from biotin containing extracts or media are intended to be purified via Strep-Tactin chromatography, biotin must be masked by the addition of avidin prior to the application onto the column. BioLock biotin blocking solution allows this masking of biotin (and biotinylated proteins) in a very fast and convenient way.Activity: >70U/mlAdd at least 1U of BioLock solution per µg of biotin.

IBA LifeSciences StrGate Acptr Vctr pESG-IBAwt1

The pESG-IBAwt1 vector is designed for high-level, stable, and non-replicative transient expression of target proteins without affinity tag in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

IBA LifeSciences StrGate Acptr Vctr pCSG-IBAwt1

pCSG-IBAwt1 is a large expression vector with universal features for transient expression as well as for generation of stable mammalian cell lines. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells and ColE1 origin for a high plasmid copy number. Extrachromosomal replication in mammalian cells could occur either by origin of replication from Epstein-Barr Virus (oriP) or by SV40 ori. For the former the vector provides the EBNA-1 gene and for the latter the cell line has to be latently infected with SV40 or express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). Stable cell lines can be selected by the neomycin resistance gene (NeoR). In addition, the human cytomegalovirus (CMV) immediate-early promoter enables a high-level expression in a wide range of mammalian cells. Besides to the direct cloning of the gene of interest into pCSG-IBA vectors with Esp3I, another option via a so-called entry vector is possible.

IBA LifeSciences StrGate Acptr Vctr pESG-IBA105

The pESG-IBA105 vector is designed for high-level, stable, and non-replicative transient expression of target proteins with an N-terminal Twin-Strep-tag in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

IBA LifeSciences StrGate Acptr Vctr pESG-IBA103

The pESG-IBA103 vector is designed for high-level, stable, and non-replicative transient expression of target proteins with a C-terminal Twin-Strep-tag in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

IBA LifeSciences StrGate Acptr Vctr pESG-IBA104

The pESG-IBA104 vector is designed for high-level, stable, and non-replicative transient expression of target proteins with an N-terminal Twin-Strep-tag in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). BM40 secretory signal peptide is encoded for the transfer of the expressed protein into the medium. During translocation from the cytosol the signal peptide is removed from the protein. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via Entry Vector is possible.

IBA LifeSciences StrGate Acptr Vctr pESG-IBAwt2

The pESG-IBAwt2 vector is designed for high-level, stable, and non-replicative transient expression of target proteins without affinity tag in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). BM40 secretory signal peptide is encoded for the transfer of the expressed protein into the medium. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

IBA LifeSciences StrGate Acptr Vctr pESG-IBA102

The pESG-IBA102 vector is designed for high-level, stable, and non-replicative transient expression of target proteins with a C-terminal Twin-Strep-tag in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). BM40 secretory signal peptide is encoded for the transfer of the expressed protein into the medium. During translocation from the cytosol the signal peptide is removed from the protein. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via Entry Vector is possible.