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LifeSensors 1,10 Phenanthroline
Supplier: LifeSensors SI96490500
1,10-phenanthroline (o-phenanthroline) is a chelator with a high affinity for divalent metal ions. While not specific for DUBs, it has been shown to be a potent inhibitor of JAMM-type isopeptidases through chelation of the active site Zn2+ ion. As such, o-phenanthroline is widely used when preparing cell lysates for the enrichment of proteins bearing K63-linked polyubiquitin chains which are especially sensitive to DUBs in the JAMM family.
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