Invitrogen™ 1,25 Dihydroxy Vitamin D3 Competitive ELISA Kit

Catalog No.	Quantity
EEL153	96 Tests

Catalog No. EEL153 Supplier Invitrogen™ Supplier No. EEL153

Description

ELISA

1,25-dihydroxy vitamin D3, or calcitriol, is the active form of vitamin D3. It functions as a hormone and plays a crucial role in regulating calcium and phosphorus metabolism. It is mainly localized in the intestines, bones, kidneys, and parathyroid glands. Structurally, it is a steroid hormone derived from cholesterol. Its functions include promoting calcium and phosphorus absorption, maintaining bone health, regulating blood calcium levels, and suppressing parathyroid hormone production.

Specifications

• Assay Range: 7.81 to 500 pg/mL
• Assay Sensitivity: 4.69 pg/mL
• Conjugate: Unlabeled
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: <10
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: 1,25 Dihydroxy Vitamin D3
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 30 mins