Brain Heart Infusion (BHI) Broth is an enrichment medium used for the cultivation of a wide variety of fastidious organisms. A rich isotonic infusion medium with tryptose (a mixture of meat and milk peptones), which provides a wide range of substrates. A low concentration of glucose is used to stimulate early growth. The medium is lightly buffered to prevent the early death of some species due to acid production. Organisms that produce significant amounts of acid may overpower the buffering system and auto-sterilize.• Product meets AOAC, BAM, CLSI, EPA, ISO, SM-Dairy, SMWW, USDA, and USP-NF reference methods and performance criteria.• For in vitro diagnostic (IVD) use.• BHI Broth should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions.