The SARS-CoV-2 S1 protein is a large transmembrane protein containing the receptor binding domain responsible for recognizing the human ACE2 receptor to facilitate cell entry. This protein is expressed from HEK293 cells, spanning amino acids Val 16 to Arg 685 of the spike glycoprotein. It contains a C-terminal polyhistidine tag and has been verified by SEC-MALS. The S1 protein plays a critical role in the induction of neutralizing-antibody and T-cell responses.
- Greater than 95% purity as determined by SDS-PAGE
- Calculated molecular weight of 76.9 kDa, migrating at 100 to 125 kDa due to glycosylation
- Endotoxin levels less than 1.0 EU per μg via LAL method
- Formulated as a lyophilized powder in PBS, pH 7.4 with trehalose
- Stable for 12 months at -20°C to -70°C in lyophilized state