Invitrogen™ 11-Ketotestosterone Competitive ELISA Kit

Catalog No.	Quantity
EIA11K	96 Tests

Catalog No. EIA11K Supplier Invitrogen™ Supplier No. EIA11K

Description

ELISA

The multispecies 11-Ketotestosterone ELISA quantitates 11-Ketotestosterone in extracted serum, plasma, and dried fecal samples, or urine. Principle of the method: The 11-Ketotestosterone Competitive ELISA research-use-only kit is designed to quantitatively measure 11-Ketotestosterone independent of species. A 11-Ketotestosterone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with goat anti-rabbit IgG antibodies. 11-Ketotestosterone polyclonal rabbit antibodies and 11-Ketotestosterone-peroxidase conjugate is added to the wells. The 11-Ketotestosterone conjugate and any 11-Ketotestosterone in the sample will compete to bind to the 11-Ketotestosterone polyclonal rabbit antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound 11-Ketotestosteron-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of 11-Ketotestosterone present in the sample. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Androgenic hormones, such as testosterone, affect many male organisms' growth, size, and reproduction. In teleost fish, along with testosterone, 11-ketotestosterone plays a significant role by inducing reproductive characteristics in both males and females. In particular male fish, 11-ketotestosterone levels increase during spermatogenesis in the spawning season, while in some female fish, 11-ketotestosterone increases before yolk deposition to regulate ovarian development. The presence and involvement of 11-ketotestosterone in other species, such as humans, have only recently been established. In contrast to fish, primate serum 11-ketotestosterone concentrations were not significantly different in males and females, despite males having significantly higher circulating testosterone. This evidence suggests that 11-ketotestosterone production in these species may not be testis-dependent and primarily originates from adrenal-derived 11-oxyandrogen precursors. Recent studies have discovered more 11-ketotestosterone than its precursors, androstenedione, and testosterone, in prepubertal children and postmenopausal women compared to men. This discovery highlights the potential use of 11-ketotestosterone as a clinical biomarker to screen adrenal androgen excess in disease conditions such as polycystic ovary syndrome (PCOS) and hirsutism in women.

Specifications

• Assay Range: 8.2-2000 pg/mL
• Assay Sensitivity: 1.85 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Chromone
• Sample Type: Fecal Extract, Plasma, Serum, Urine
• Target Species: Chemical
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 11KT; 11-KT11KT; 11-KT;
• Interassay CV: <8%
• Intraassay CV: <6%
• Kit Contents: Pre-coated 96 well plate, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers, Standard, Antibody, HRP-Conjugated Detection Antibody, Assay Buffer Concentrate, Wash Buffer, TMB Substrate, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Protein Aliases: 17-Hydroxyandrost-4-ene-3,11-dione,11-Ketotestosterone, 11 Oxotestosterone
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Urine 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: 2-8°C
• Target: 11-Ketotestosterone
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min