Bacteroides Bile Esculin (BBE) Agar is an enriched, selective and differential medium recommended for the isolation and presumptive identification of gram-negative obligate anaerobes, such as Bacteroides fragilis and Bilophila species. Selective inhibition of facultative anaerobes and most gram-negative anaerobic organisms is obtained by the presence of gentamicin and oxgall. Differentiation of the B. fragilis is based on esculin hydrolysis, which produces esculetin and dextrose. The esculetin reacts with the iron salt contained in the medium to produce a dark brown to black complex that appears in the medium surrounding colonies of members of the B. fragilis.• Product meets CLSI reference methods and performance criteria.• BBE Agar should be used by trained professionals within a laboratory setting. • For in vitro diagnostic (IVD) use.• Product is not intended for use in the diagnosis or treatment of disease or other human conditions.