Modified Letheen Agar with Tween 80 (MLAT) is a non-selective medium utilized to determine the antimicrobial activity of quaternary ammoniums compounds and is recommended for the microbiological testing of cosmetics. Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers in 1948. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralized quaternary ammonium compounds to test germicidal activity. Total neutralization of disinfectants is critical as disinfectant residues can result in false negative (no-growth) test results. • Product meets APHA, BAM, CLSI, SM-Dairy, SMWW, and USP-NF reference methods and performance criteria.• MLTA should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions.