Invitrogen™ 17-Hydroxyprogesterone Competitive ELISA Kit

Catalog No.	Quantity
EIA17OHPX10	10 x 96 Tests
EIA17OHP	96 Tests

Catalog No. EIA17OHPX10 Supplier Invitrogen™ Supplier No. EIA17OHPX10

Description

ELISA

The 17-Hydroxyprogesterone Competitive ELISA quantitates 17-Hydroxyprogesterone in serum,plasma,urine,dried fecal extracts or cell culture medium.

Principle of the method

The 17-Hydroxyprogesterone Competitive ELISA research-use-only kit uses a specifically generated antibody to measure 17-Hydroxyprogesterone (17HO-P) and its metabolites in reconstituted buffer samples,tissue culture media samples,urine and fecal samples,or in extracted serum and plasma. This kit is not recommended for serum or plasma samples without extraction. A 17HO-P standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into the provided plate coated with a capture antibody. A 17HO-P-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to 17HO-P to each well. After a 1-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound 17HO-P-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.

Rigorous validation

Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.

17-Hydroxyprogesterone (4-pregen-17-ol-3, 20-dione, 17HO-P, OHPG) is a steroid hormone from the androgen group that is found in mammals, reptiles, birds, and other vertebrates. 17HO-P is identical across all species and so this kit should measure 17HO-P from all sources. In humans, it is primarily synthesized in the adrenal glands, the gonads, testes, and placenta.

Specifications

• Assay Range: 24.69 to 6000 pg/mL
• Assay Sensitivity: 20.3 pg/mL
• Description: 17-Hydroxyprogesterone Competitive ELISA Kit
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Fecal Extract, Plasma, Serum, Supernatant, Urine
• Sufficient For: 10 x 96 Assays
• For Use With (Application): ELISA, Protein Assays, Aktivity Assays, Protein Biology, Reproductive Biology, Inflammation, Oxidative Stress, Metabolism, Cell Signaling, Epigenetics, Hematology, DNA Damage, Cancer Biology, Immunology, Neuroscience, Neurobiology, Steroid Hormone Detecti
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 8%
• Intraassay CV: 6.6
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, HRP-Conjugated Detection Antibody, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Reproductive Biology
• Sample Volume: Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 25 μL
• Storage Requirements: -20°C
• Target: 17-Hydroxyprogesterone
• Test Time: 1 hrs 20 mins
• Total Assay Time: 1 hrs 30 mins