Abnova™ 19q13/19p13 CISH Probe

Catalog No.	For Use With (Application)
89-335-530	CISH-P

Catalog No. 89-335-530 Supplier Abnova™ Supplier No. CG0001

Description

19q13/19p13 CISH Probe is designed for the qualitative detection of human chromosome 19q13.32-q13.33 and 19p13.3 specific sequences in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as dark green colored distinct dots (19p13 region), and dinitrophenyl-labeled polynucleotides appear as bright red colored distinct dots (19q13 region).
Normal situation: In interphases of normal cells or cells without aberrations of chromosome 19, two red (19q13) signals and two green (19p13) signals appear.
Aberrant situation: In a cell with deletions affecting the 19q13 locus, one or no copy of the red signal will be observed.
Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• For Use With (Application): CISH-P
• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.