Enzo Life Sciences Proteasome 19S Rpn10 / S5a subunit (human), (recombinant) (GST-tag) (100 µg)

Supplier:  Enzo Life Sciences BMLUW84650100

A subunit of the 26S proteasome, S5a, recognises and binds multi-ubiquitinylated proteins containing chains of at least four ubiquitin moieties. Purified recombinant S5a retains the ability to bind multi-ubiquitinylated proteins in isolation, when immobilised on glutathione-Sepharose, and after being eluted from glutathione-Sepharose, run on SDS-PAGE, and blotted onto nitrocellulose. Human S5a sequence, with an N-terminal fusion of glutathione S-transferase (Schistosoma japonicum), was expressed in E. coli with GST tag at amino terminus. A subunit of the 26S proteasome, S5a, recognises and binds multi-ubiquitinylated proteins containing chains of at least four ubiquitin moieties. Alternative name: 26S proteasome non-ATPase regulatory subunit 4, Antisecretory factor 1, Multiubiquitin chain-binding protein. Purity: ≥95% (SDS-PAGE). Formulation: Liquid. In 20mM TRIS, pH 7.5, containing 500mM sodium chloride. Source: Produced in E. coli.

Catalog No. 50-201-2281