2',7'-Dichlorofluorescein, Thermo Scientific Chemicals

Description

2',7'-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
2′,7′-Dichlorofluorescein is used as an indicator and as a cellular antioxidant activity. It is also used for the determination of carbohydrates and hydrogen peroxide by fluorescence densitometry and chemiluminescence respectively. It is used as an indicator for argentometry by Fajans method. It is also involved in the cellular antioxidant activity assay. It is utilized as an additive in fluorescence and laser studies.

Solubility
Soluble in ethanol and methanol. Slightly dimethyl sulfoxide. Insoluble in water.

Notes
As a fluorescent indicatorIncompatible with strong oxidizing agents.

Specifications

Chemical Identifiers

• CAS: 76-54-0
• Molecular Formula: C20H10Cl2O5
• Molecular Weight (g/mol): 401.195
• MDL Number: MFCD00005047
• InChI Key: VFNKZQNIXUFLBC-UHFFFAOYSA-N
• Synonym: 2',7'-dichlorofluorescein, dichlorofluorescein, unii-56nqm5uzt1, 2,7-dichlorofluorescein, 56nqm5uzt1, spiro isobenzofuran-1 3h ,9'-9h xanthen-3-one, 2',7'-dichloro-3',6'-dihydroxy, 2',7'-dichloro-3',6'-dihydroxy-3h-spiro isobenzofuran-1,9'-xanthen-3-one, 2',7'-dichloro-3',6'-dihydroxyspiro 2-benzofuran-3,9'-xanthene-1-one, 11,16-dichloro-12,15-dihydroxyspiro 3-hydroisobenzofuran-3,9'-xanthene-1-one
• PubChem CID: 64944
• ChEBI: CHEBI:51596
• IUPAC Name: 2',7'-dichloro-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
• SMILES: C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C=C4OC5=CC(=C(C=C35)Cl)O)O)Cl

• Melting Point: >300°C (decomposition)
• Quantity: 25 g
• Solubility Information: Soluble in ethanol and methanol. Slightly dimethyl sulfoxide. Insoluble in water.
• Formula Weight: 401.2
• Chemical Name or Material: 2',7'-Dichlorofluorescein

Safety and Handling

Hazard Category	H315-H319-H335-H500
Hazard Statement	GHS H Statement H319 Causes serious eye irritation.
Precautionary Statement	P261-P264b-P271-P280-P302+P352-P304+P340-P305+P351+P338-P312-P332+P313-P362-P501c
EINECSNumber	200-968-6
TSCA	Yes
Recommended Storage	Ambient temperatures

SDS

RUO – Research Use Only