Sigma Aldrich Fine Chemicals Biosciences 2 D Clean Up Kit Cytiva 80

Supplier:  Sigma Aldrich Fine Chemicals Biosciences GE80648451

2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to highconductivity high levels of interfering substances or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances such as detergents salts lipids phenolics and nucleic acids in SOLUTIONon.Treatment of the sample with 2-D Clean-Up Kit can improve the quality of 2-D electrophoresis results reducing streaking background staining and other artifacts due to interfering contaminants. The kit can make 2-D analysis possible for samples that are otherwise too dirty or too dilute.The 2-D Clean-Up Kit procedure uses precipitant and co-precipitant in combination to quantitatively precipitate proteins. Precipitated proteins are pelleted by centrifugation and the precipitate is further washed to remove nonprotein contaminants. After a second centrifugation the resultant pellet is resuspended into denaturing sample

Catalog No. 50-290-4251