Invitrogen™ 20-Hydroxyecdysone ELISA Kit

Catalog No.	Quantity
EIAHYD	96 Tests
EIAHYDX10	10 x 96 Tests

Catalog No. EIAHYD Supplier Invitrogen™ Supplier No. EIAHYD

Description

ELISA

The 20-Hydroxyecdysone Competitive ELISA quantitates 20-Hydroxyecdysone (20E) in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.∣Principle of the method: The 20-Hydroxyecdysone Competitive ELISA research-use-only kit is designed to quantitatively measure 20-Hydroxyecdysone independent of species. A 20-Hydroxyecdysone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit 20-Hydroxyecdysone antibodies and 20-Hydroxyecdysone-peroxidase conjugate is added to the wells. The 20-Hydroxyecdysone-peroxidase conjugate and any 20-Hydroxyecdysone in the sample will compete to bind to the anti-rabbit 20-Hydroxyecdysone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound 20-Hydroxyecdysone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of 20-Hydroxyecdysone present in the sample.∣Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

20-Hydroxyecdysone is the most widely occurring ecdysteroid in both plant and animal species. It controls cell death during metamorphosis of Drosophila melanogaster.

Specifications

• Accession Number: 20 E; C27H44O7; ecdysterone
• Assay Range: 102.4 to 25,000 pg/mL
• Assay Sensitivity: 197 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Fecal Extract, Plasma, Serum, Supernatant, Urine
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 9%
• Intraassay CV: 10
• Kit Contents: Anti-IgG coated 96 well plate, Standard, Assay Buffer Concentrate, Capture Antibody, HRP-conjugated Antigen, Wash Buffer Concentrate, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 12.5 μL
• Storage Requirements: -20°C
• Target: 20-Hydroxyecdysone
• Test Time: 45 mins
• Total Assay Time: 2 hrs 30 mins