Recombinant Protein;200UG;Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (SPD-C82E9) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1).;This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 28.2 kDa. The protein migrates as 33-36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) with a linear range of 4-125 ng/mL (QC tested).;Immobilized Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.1-3 ng/mL (Routinely tested).