Invitrogen™ 20X Bolt™ MES SDS Running Buffer

Catalog No.	Quantity
B0002	500 mL
B000202	5 L

Catalog No. B0002 Supplier Invitrogen™ Supplier No. B0002

Description

Optimized for use with Bolt™ Bis-Tris Plus gels, and are available in a variety of formats

About Bolt™ Bis-Tris Plus Gels
Bolt™ Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE™ Bis-Tris gels, Bolt™ Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt™ gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt™ gels to obtain optimal results for your day-to-day protein separation needs. Bolt™ Bis-Tris Plus gels come in four gel types and multiple well formats. A Mini Gel Tank is required for use of all Bolt™ gels.

Bolt™ gel run times may vary depending upon the type of running buffer used.

Order Info

Shelf-life is 6 months, when stored properly.

Specifications

• Chemical Name or Material: MES SDS Running Buffer
• Concentration: 20X
• For Use With (Equipment): Bolt
• Product Line: Bolt
• Quantity: 500 mL

Frequently Asked Questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Which power supplies are compatible with the Mini Gel Tank?

The most common power supplies from Thermo Fisher Scientific, Bio-Rad, and Hoefer are compatible. Also, power supply adapters are available for power supplies not designed for use with covered or non-retractable power leads. Thermo Fisher Owl branded gel tanks are not compatible

Are Bolt gels compatible with transfer devices from other suppliers?

Yes. While we would prefer that you use our devices, Bolt gels can also be transferred using devices from Bio-Rad, including the Mini Trans-Blot Cell, Trans-Blot SD Semi-Dry Transfer Cell, or Trans-Blot Turbo Transfer System.

Are Bolt gels compatible with other transfer devices?

Yes. You can use the iBlot Dry Transfer System or XCell II Blot Module with SureLock tank and achieve similar transfer efficiency.

Is there a difference in composition between the NuPAGE MES SDS Running Buffer (20X) and the 20X Bolt MES SDS Running Buffer?

Both the NuPAGE MES SDS Running Buffer (20X) (Cat. No. NP0002) and the 20X Bolt MES SDS Running Buffer (Cat. No. B0002) have the exact same composition: 50 mM MES, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.3.

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.