Invitrogen™ 25-Hydroxy Vitamin D3 Competitive ELISA Kit

Catalog No.	Quantity
EEL152	96 Tests

Catalog No. EEL152 Supplier Invitrogen™ Supplier No. EEL152

Description

ELISA

The 25 Hydroxy Vitamin D3 (25 HVD3) ELISA quantitates 25 HVD3 in serum, plasma and other biological fluids. Principle of the method The competitive multispecies ELISA research use only kit is designed to quantitatively measure 25-Hydroxy Vitamin D3 (25-HVD3), independent of species . A 25-HVD3 standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to 25-Hydroxy Vitamin D3 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. A TMB substrate solution is then added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of 25-HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

25-OH Vitamin D3 (calcifediol) is a prohormone produced by the liver by hydroxylation of vitamin D3 and is used to measure vitamin D status and detect itamin D deficiency.

Specifications

• Assay Range: 3.13-200 ng/mL
• Assay Sensitivity: 1.88 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 25-hydroxy vitamin D; 25-hydroxyvitamin D; 25-OH Vitamin D25-hydroxy vitamin D; 25-hydroxyvitamin D; 25-OH Vitamin D;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: 25-OH Vitamin D3
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min