This Master Mix contains Es Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer. The concentration is 2x.Es Taq DNA Polymerase possesses 5´→3´ DNA polymerase and 5´→3´ exonuclease activity. The polymerase has the high amplification efficiency and low mismatching as Taq DNA polymerase and high fidelity of Pfu DNA polymerase. Es Taq Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for T/A cloning. The amplification range of Es Taq is ~ 6 kb.This unique Master Mix recipe makes the system very reliable. More than 98% of PCR reaction can get successful amplification during the first try. It also works well on complicate templates.The Master Mix contains dye, and can directly run electrophoresis after PCR reaction.