3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity, Thermo Scientific Chemicals

Description

3,3’,5,5’-Tetramethylbenzidine (TMB, CAS # 54827-17-7) is a blue chromogenic substance used in staining techniques such as immunohistochemistry.

TMB is a substrate for horseradish peroxidase. Recommended for ELISA (microwell) procedures,3,3?,5,5?-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3?,5,5?-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• TMB is an aromatic amine that acts as a hydrogen donor in horseradish peroxidase-catalyzed reactions
• The transfer of hydrogen results in a blue stain associated with the target of interest
• This pH-dependent color change can be confirmed using spectrophotometric methods
• TMB is commonly used in immunohistochemistry; TMB is light-sensitive, and all applications should be performed in the dark

Application

• TMB is suitable for immunohistochemistry and immunocytochemistry applications including ELISA and cell and tissue staining

Specifications

Chemical Identifiers

• CAS: 54827-17-7
• Molecular Formula: C16H20N2
• Molecular Weight (g/mol): 240.35
• MDL Number: MFCD00007748
• InChI Key: UAIUNKRWKOVEES-UHFFFAOYSA-N
• Synonym: 3,3',5,5'-tetramethylbenzidine, 3,3',5,5'-tetramethyl-1,1'-biphenyl-4,4'-diamine, 3,5,3',5'-tetramethylbenzidine, tmb, 3,3',5,5'-tetramethyl benzidine, tmb substrate, bm blue, ccris 4727, tmb plus, tmb, insoluble
• PubChem CID: 41206
• IUPAC Name: 4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
• SMILES: CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C

• Color: Yellow
• Quantity: 125 mL
• UN Number: UN2810
• Beilstein: 2808541
• Solubility Information: Insoluble in water. Soluble in organic solvents such as DMSO,96 % Ethanol,Acetone,Chloroform,Toluene.
• Formula Weight: 240.35
• Physical Form: Liquid
• Chemical Name or Material: 3,3',5,5'-Tetramethylbenzidine soln., Ready-to-Use, high sensitivity

Safety and Handling

Hazard Category	H226-H302+H312-H331-H336-H370
Hazard Statement	GHS H Statement H370-H312-H332 Causes damage to organs. Harmful in contact with skin. Harmful if inhaled.
Precautionary Statement	P210-P233-P235-P240-P241-P242-P243-P260-P264b-P270-P271-P280-P301+P312-P303+P361+P353-P304+P340-P307+P311-P311-P312-P330-P363-P370+P378q-P501c
DOTInformation	Transport Hazard Class: 6.1; Packing Group: III; Proper Shipping Name: TOXIC, LIQUIDS, ORGANIC, N.O.S.
EINECSNumber	259-364-6
TSCA	Yes
Recommended Storage	Keep cold

SDS

RUO – Research Use Only