3-Hydroxyflavone (CAS No 577-85-5) is a bioactive flavonoid extensively studied in biomedical research for its antioxidant and anti-inflammatory properties Originating from natural sources like fruits and vegetables it functions by modulating signaling pathways including MAPK and NF- B influencing cellular responses such as apoptosis and oxidative stress reduction Its in vitro activity generally falls within the nanomolar to low micromolar range showcasing efficacy in diverse biological experiments Commonly applied in cell models such as cancer cell lines and neuroprotective studies 3-hydroxyflavone is also utilized in animal models to explore therapeutic potentials against degenerative diseases It serves as a promising candidate in drug screening assays due to its ability to interact with multiple cellular targets Experimental concentrations typically depend on the specific research design aiming to achieve optimal biological impact while minimizing toxicity