3-Methylpyrazole (CAS No 1453-58-3) is a compound widely used in biomedical research particularly in the study of enzyme inhibition and metabolic pathways It acts as a potent inhibitor of alcohol dehydrogenase which is crucial for the metabolism of alcohol in the liver The compound shows inhibitory activity in the nanomolar to low micromolar range making it effective in various biochemical assays It is commonly utilized in in vitro studies with cell models to explore metabolic processes and assess toxicity Additionally animal studies leverage its inhibitory effects to investigate potential treatments for conditions such as methanol poisoning Experimental concentrations typically depend on the specific research design and it is often employed in drug screening assays to evaluate therapeutic efficacy and safety 3-Methylpyrazole s role as a research tool provides valuable insights into metabolic disorders and enzymatic mechanisms