Enzo Life Sciences BIO-PROBE 3'-Oligonucleotide labeling system reagent pack (25Reactions)

Supplier:  Enzo Life Sciences ENZ42730

The procedure for labeling of DNA probes with a polynucleotide "tail" containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions. Terminal labeling is an ideal procedure for the labeling of oligonucleotides. An oligonucleotide can be synthesized using standard, commercially available reagents and labeled after synthesis in a simple and reproducible enzyme reaction. The BIO-PROBE 3'-Oligonucleotide Labeling System provides maximum flexibility for labeling oligonucleotides approximately 15 to 100 nucleotides in length. A standard reaction will label up to 100 picomoles. Applications: Southern Blot, Northern Blot, ISH (in situ hybridization), Dot Blot.

Catalog No. 50-201-2535