Applied Biosystems™ Sequencing Standard, BigDye™ Terminator v1.1 Dx

Catalog No.	Quantity
44-621-13	4 Tubes

Catalog No. 44-621-13 Supplier Applied Biosystems™ Supplier No. 4462113

Description

Includes

This kit contains four tubes of DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v1.1. Each tube can perform one spectral calibration/performance check and/or one control sequencing run on the Applied Biosystems™ Dx Series Genetic Analyzers

Sufficient DNA is included to perform one spectral calibration/performance check or one control sequencing run on Applied Biosystems Dx genetic analyzers.

Sequencing Standard, BigDye Terminator v1.1 Dx, consists of four tubes, each of which contains DNA of a known 1,200 base pair sequence prepared with BigDye Terminator v1.1. Sufficient DNA is included to perform one spectral calibration/performance check and/or one control sequencing run on an Applied Biosystems Dx genetic analyzer. The DNA preparation has been lyophilized to maximize stability.

Note: Applied Biosystems Dx genetic analyzers and consumables are for distribution and use in specific European countries only. Not for use in the USA.

This BigDye® Terminator v1.1 sequencing standard consists of four tubes, each of which contains DNA of a known 1,200 base pair sequence prepared with BigDye® Terminator v1.1.

Order Info

Each

Specifications

• Content And Storage: This kit contains four tubes of DNA of a known 1200 base pair sequence prepared with BigDye™ Terminator v1.1. Each tube can perform one spectral calibration/performance check and/or one control sequencing run on the Applied Biosystems 3500 Dx Series Genetic Analyzers. Store kit at -15 to -25°C.
• For Use With (Equipment): 3500xL Dx Genetic Analyzer, 3500 Dx Genetic Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer
• For Use With (Application): Sequencing
• Label or Dye: ROX, TAMRA™, Rhodamine 110, Rhodamine 6G
• Product Line: BigDye™ Terminator
• Product Type: Sequencing Standard
• Quantity: 4 Tubes

Frequently Asked Questions (FAQs)

When I started my run on the Applied Biosystems 3500/3500xL Genetic Analyzer, an error occurred: "Unstable Electrophoresis current detected, check for air bubbles" but I don't see any bubbles. What else can cause this?

Some of the other causes of an Unstable Electrophoresis current detected error message are:

1.Leak on the system
2. Polymer that:
-Has expired
-Has been left on the instrument for more than the recommended time
-Is a mixture of expired polymer and non-expired polymer
3. Running Buffer that was:
-On the instrument longer than 2 weeks
-Not filled to the fill line or evaporated below the fill line
4. An arcing event that was not cleaned afterwards using the water wash wizard
5. Not performing regular maintenance on the instrument
6. Hardware issues

Inspect the system for leaks. If you do not see any leaks on the system, perform the wash the pump chamber and channels wizard using the conditioning pouch and place fresh, non-expired polymer and fresh Anode and Cathode buffer containers. If the problem persists, a service call may be required.

Do I need to replace the buffer every 14 days if the Applied Biosystems 3500/3500xL Genetic Analyzer is not in use?

If the instrument is not in use, it is not necessary to replace the buffer every 14 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer containers prior to starting a new run.

For In Vitro Diagnostic Use Only