3-D Life is the most flexible system for biomimetic hydrogel design. It uses two polymers that form a hydrogel by covalent crosslinking at ambient temperature. Chemical reaction is non-toxic to cells and occurs rapidly, preventing cell settlement before gel is formed. 3-D Life Dextran-CD Hydrogel SG Kit provides reagents to set up slow gelling, cell-compatible hydrogels for 3D cell cultures. Thiol groups on crosslinker CD-Link form stable thioether bonds with thiol-reactive groups on SG-Dextran, resulting in gel formation. Mixed at physiological pH 7.2 for optimal cell compatibility. CD-Link is designed for MMP cleavage, allowing cell spreading/migration if they express the indicated MMPs. Cell adhesion peptides can be attached to SG-Dextran to provide a cell-adhesive matrix. Dextran hydrogels can be dissolved by adding dextranase, allowing easy cell recovery. One kit allows formation of up to 2 ml hydrogel depending on gel stiffness. Manufactured in Germany by Cellendes GmbH.