3-D Life is the most flexible system for biomimetic hydrogel design for 3D cell cultures. It uses two polymers that form a hydrogel by covalent crosslinking at ambient temperature. The chemical reaction is non-toxic to cells and occurs rapidly, preventing cell settlement before gel is formed. 3-D Life Dextran-HA Hydrogel Kit enables set up of hydrogels containing hyaluronic acid (hyaluronan). Thiol groups on crosslinker HyLink form stable thioether bonds with thiol-reactive groups on SG-Dextran, resulting in gel formation. Mixed at physiological pH 7.2 for optimal cell compatibility. Slow gelation allows for manipulation before onset of gel formation. Cell adhesion peptides (RGD, Cat. No. 09-P-001) can be attached to SG-Dextran to provide a cell-adhesive matrix. Dextran hydrogels can be dissolved by dextranase (Cat. No. D10-1), allowing easy cell recovery. One kit allows formation of 3.3 ml hydrogel of a crosslinking strength of 1.2 mmol/L. Manufactured in Germany by Cellendes GmbH.