The lung epithelium undergoes a complex series of endodermndashmesoderm-mediated signaling events to generate a complex arborized network of conducting airway (bronchi bronchioles) and gas-exchanging units (alveoli). Lung epithelium differentiation stages include anterior/posterior and dorsal/ventral patterning lung specification lung budding branching morphogenesis and maturation. The 3dGRO(TM) Lung Organoid Branching Medium is a chemically defined/serum-free ready-to-use media used to differentiate Sox2/Pax6 anterior foregut endoderm (AfE) cells into lung bud organoids in suspension cultures. Additionally the 3dGRO(TM) Lung Organoid Branching Medium can be used to culture AfE cells on fibronectin coated cultureware. The 3dGRO(TM) Lung Organoid Branching Medium is a component of the 3dGRO(TM) Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar s