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Biotium 5-(And-6)-Carboxyfluorescein
Supplier: Biotium 51013
5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator. Its excitation spectrum and fluorescence response to pH are similar to those of BCECF. Carboxyfluorescein can also be used as a tracer dye. The dye is membrane-impermeant and can be loaded into cells by microinjection or scrape loading. Properties: Ex/Em (pH 9) = 492/514 nm; e (pH 9) = 82,000; Yellow orange solid soluble in pH>6 water; Store at 4°C and protect from light, especially in solution; C21H12O7; MW: 376; [72088-94-9].
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