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AdipoGen 5-Bromo-2-deoxyuridine
Supplier: AdipoGen CDXB0301G005
Chemical. CAS 59-14-3. Formula C9H11BrN2O5. MW 307.1. The synthetic nucleoside 5-Bromo-2-deoxyuridine BrdU is a thymidine analog used to label DNA and commonly used in the detection of proliferating cells in living tissues. It can be incorporated into newly synthesized DNA in place of thymidine during the S phase of the cell cycle. Cells that were actively proliferating can then be detected by denaturing the DNA and allowing specific antibodies with fluorescent tags to target the BrdU incorporation for detection via flow cytometry or fluorescence microscopy. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat. BrdU is also used as a mutagen in genetic research. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells.
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