5-Bromo-3-indolyl beta-d-galactopyranoside, 98+%

Description

alpha-Galactosidase substrate for Lac-gene detection systems | CAS: 97753-82-7 | C14H16BrNO6 | 374.19 g/mol

5-Bromo-3-indolyl-β-D-galactopyranoside is an α-galactosidase substrate which is converted to an insoluble indigo-blue chromophore darker than that released by X-GAL. It is ideal for Lac gene detection systems in immunoblotting, immunocytochemical, and histological applications. Also used as chromogenic substrate that is suitable for identification of lac-(+) bacterial colonies.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
5-Bromo-3-indolyl-β-D-galactopyranoside is an α-galactosidase substrate which is converted to an insoluble indigo-blue chromophore darker than that released by X-GAL. It is ideal for Lac gene detection systems in immunoblotting, immunocytochemical, and histological applications. Also used as chromogenic substrate that is suitable for identification of lac-(+) bacterial colonies.

Solubility
Soluble in dimethylformamide (100 mg/ml), dimethylformamide/water (1:1 v/v) (10 mg/ml - clear, practically colorless solution).

Notes
Store in freezer (-20°C). Keep the container tightly closed. Store away from oxidizing agent.

Specifications

Chemical Identifiers

• CAS: 97753-82-7
• Molecular Formula: C14H16BrNO6
• Molecular Weight (g/mol): 374.19
• MDL Number: MFCD00063691
• InChI Key: LINMATFDVHBYOS-MBJXGIAVSA-N
• Synonym: 5-bromo-3-indolyl-beta-d-galactopyranoside, 5-bromo-3-indolyl-b-d-galactopyranoside, bluo-gal, blue-gal, 2s,3r,4s,5r,6r-2-5-bromo-1h-indol-3-yl oxy-6-hydroxymethyl oxane-3,4,5-triol, 5-brig, blue gal, 5-bromo-3-indolyl-beta-galactoside, 5-bromo-3-indolyl beta-d-galactopyranoside, 5-bromo-3-indoxyl-beta-d-galactopyranoside
• PubChem CID: 126898
• IUPAC Name: (2S,3R,4S,5R,6R)-2-[(5-bromo-1H-indol-3-yl)oxy]-6-(hydroxymethyl)oxane-3,4,5-triol
• SMILES: OC[C@H]1O[C@@H](OC2=CNC3=CC=C(Br)C=C23)[C@H](O)[C@@H](O)[C@H]1O

• Color: White
• Quantity: 100 mg
• Solubility Information: Soluble in dimethylformamide (100mg/ml),dimethylformamide/water (1:1 v/v) (10mg/ml- clear,practically colorless solution).
• Formula Weight: 374.18
• Percent Purity: ≥98%
• Physical Form: Powder
• Chemical Name or Material: 5-Bromo-3-indolyl beta-d-galactopyranoside

Safety and Handling

TSCA	No
Recommended Storage	Store at -20°C

SDS

RUO – Research Use Only