5-Bromo-4-chloro-3-indolyl phosphate disodium salt, 98+%, Thermo Scientific Chemicals

Description

It is an histochemical substrate for alkaline phosphatase. For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
It is an histochemical substrate for alkaline phosphatase. For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

Solubility
Soluble in water and toluene.

Notes
Moisture and light sensitive. Store in dry place. Keep away from oxidizing agents, heat and light.

Specifications

Chemical Identifiers

• CAS: 102185-33-1
• Molecular Formula: C8H4BrClNNa2O4P
• Molecular Weight (g/mol): 370.43
• MDL Number: MFCD00036757
• InChI Key: OAZUOCJOEUNDEK-UHFFFAOYSA-L
• PubChem CID: 6097197
• IUPAC Name: disodium;(5-bromo-4-chloro-1H-indol-3-yl) phosphate
• SMILES: [Na+].[Na+].[O-]P([O-])(=O)OC1=CNC2=CC=C(Br)C(Cl)=C12

• Quantity: 1 g
• Beilstein: 4722243
• Sensitivity: Light and moisture sensitive
• Synonym: sodium 5-bromo-4-chloro-1h-indol-3-yl phosphate, 5-bromo-4-chloro-3-indolyl phosphate disodium salt, 5-bromo-4-chloro-3-indoxyl phosphate, disodium salt, bcip™, 1h-indol-3-ol, 5-bromo-4-chloro-, 3-dihydrogen phosphate , sodium salt 1:2, disodium 5-bromo-4-chloro-1h-indol-3-yl phosphate, 1h-indol-3-ol,5-bromo-4-chloro-, 3-dihydrogen phosphate , sodium salt 1:2, 5-bromo-4-chloro-3-indolyl phosphate, 5-bromo-4-chloro-3-indolylphosphate disodium salt
• Solubility Information: Soluble in water and toluene.
• Formula Weight: 370.43
• Percent Purity: ≥98%
• Chemical Name or Material: 5-Bromo-4-chloro-3-indolyl phosphate disodium salt

Safety and Handling

TSCA	No
Recommended Storage	Store at -20°C

SDS

RUO – Research Use Only