5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt, MP Biomedicals™

Description

Used as a chromogenic substrate for alkaline phosphatase. 5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt, MP Biomedicals (BCIP) provides colormetric detection of alkaline-phosphatase-labeled molecules.

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available.

• The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry
• BCIP disodium salt is soluble in water. It may be used to prepare a stock solution, which in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase
• This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color.

Specifications

Chemical Identifiers

• CAS: 102185-33-1
• Molecular Formula: C8H4BrClNNa2O4P
• Molecular Weight (g/mol): 370.43
• MDL Number: MFCD00036757
• InChI Key: OAZUOCJOEUNDEK-UHFFFAOYSA-L
• PubChem CID: 6097197
• IUPAC Name: disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate
• SMILES: [Na+].[Na+].[O-]P([O-])(=O)OC1=CNC2=CC=C(Br)C(Cl)=C12

• Melting Point: >300°C (literature)
• Quantity: 50 mg
• Synonym: sodium 5-bromo-4-chloro-1h-indol-3-yl phosphate, 5-bromo-4-chloro-3-indolyl phosphate disodium salt, 5-bromo-4-chloro-3-indoxyl phosphate, disodium salt, bcip™, 1h-indol-3-ol, 5-bromo-4-chloro-, 3-dihydrogen phosphate , sodium salt 1:2, disodium 5-bromo-4-chloro-1h-indol-3-yl phosphate, 1h-indol-3-ol,5-bromo-4-chloro-, 3-dihydrogen phosphate , sodium salt 1:2, 5-bromo-4-chloro-3-indolyl phosphate, 5-bromo-4-chloro-3-indolylphosphate disodium salt
• Solubility Information: Disodium salt is soluble in water (50mg/mL); insoluble in dimethylformamide.
• Formula Weight: 370.4
• Percent Purity: ≥99%
• Physical Form: Powder

Safety and Handling

Recommended Storage

−20°C, desiccate, protect from light