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  7. 5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt, MP Biomedicals™

5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt, MP Biomedicals™

Catalog No. MP215004291 Shop All MP Biomedicals, Inc Products
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Description

Used as a chromogenic substrate for alkaline phosphatase. 5-Bromo-4-Chloro-3-Indolyl Phosphate Disodium Salt, MP Biomedicals (BCIP) provides colormetric detection of alkaline-phosphatase-labeled molecules.

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available.

  • The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry
  • BCIP disodium salt is soluble in water. It may be used to prepare a stock solution, which in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase
  • This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color.
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 102185-33-1
Molecular Formula C8H4BrClNNa2O4P
Molecular Weight (g/mol) 370.43
MDL Number MFCD00036757
InChI Key OAZUOCJOEUNDEK-UHFFFAOYSA-L
PubChem CID 6097197
IUPAC Name disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate
SMILES [Na+].[Na+].[O-]P([O-])(=O)OC1=CNC2=CC=C(Br)C(Cl)=C12

Specifications

Melting Point >300°C (literature)
Quantity 1 g
Synonym sodium 5-bromo-4-chloro-1h-indol-3-yl phosphate, 5-bromo-4-chloro-3-indolyl phosphate disodium salt, 5-bromo-4-chloro-3-indoxyl phosphate, disodium salt, bcip™, 1h-indol-3-ol, 5-bromo-4-chloro-, 3-dihydrogen phosphate , sodium salt 1:2, disodium 5-bromo-4-chloro-1h-indol-3-yl phosphate, 1h-indol-3-ol,5-bromo-4-chloro-, 3-dihydrogen phosphate , sodium salt 1:2, 5-bromo-4-chloro-3-indolyl phosphate, 5-bromo-4-chloro-3-indolylphosphate disodium salt
Solubility Information Disodium salt is soluble in water (50mg/mL); insoluble in dimethylformamide.
Formula Weight 370.4
Percent Purity ≥99%
Physical Form Powder

Safety and Handling

Recommended Storage −20°C, desiccate, protect from light
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