Invitrogen™ 5-HIAA Competitive ELISA Kit

Catalog No.	Quantity
EEL161	96 Tests

Catalog No. EEL161 Supplier Invitrogen™ Supplier No. EEL161

Description

ELISA

The 5 Hydroxyindoleacetic Acid (5 HIAA) ELISA quantitates 5 HIAA in serum, plasma and other biological fluids. Principle of the method The Competitive Gastrin (GT) ELISA research use only kit is designed to quantitatively measure GT in Humans. A GT standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Human GT is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Human GT in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

5-Hydroxyindoleacetic acid (5-HIAA) is the primary metabolite of serotonin (5-HT) and serves as a key marker for serotonin metabolism. As a more stable compound than serotonin, 5-HIAA is often measured in clinical settings to assess serotonin production and turnover. This measurement typically involves analyzing urine or plasma samples. Elevated levels of 5-HIAA in the urine can indicate various conditions such as carcinoid tumors, which are known to secrete large amounts of serotonin. Additionally, 5-HIAA has been implicated in inflammatory processes, notably acting as a potent ligand for the GPR35 receptor to promote neutrophil recruitment to inflamed tissues. The presence of 5-HIAA in cerebrospinal fluid is also used as a biomarker in neurological diseases impacting dopaminergic and serotoninergic pathways, although its levels can be influenced by age and other factors. The clinical relevance of 5-HIAA underscores its utility in diagnosing and monitoring various health conditions, particularly those involving serotonin dysregulation.

Specifications

• Assay Range: 1.56-100 ng/mL
• Assay Sensitivity: 0.94 ng/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 5-Hydroxyindoleacetic acid; urine5-Hydroxyindoleacetic acid; urine;
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: 5-HIAA
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min