Tryptic Soy Broth with 1% Polysorbate 80 (TSB w/T) with a septum cap is a non-selective and non-differential general growth medium used in for the detection and recovery of microorganisms, particularly in the presence of antimicrobial agents. The addition of Polysorbate 80 (Tween 80) serves as a neutralizer, inactivating residual disinfectants or preservatives that could otherwise inhibit microbial growth. This formulation is frequently employed in sterility testing, environmental monitoring, disinfectant efficacy studies, and microbial limits testing of non-sterile products to ensure accurate detection of contaminants.• Product base meets AOAC, BAM, EP, ISO, SM-Dairy, SMWW, USDA, and USP-NF reference methods and performance criteria.• TSB should be used by trained professionals within a laboratory setting. • Product is not intended for use in the diagnosis or treatment of disease or other human conditions.• Septum Cap