Recombinant Protein;200UG;Biotinylated Human B7-H3 (4Ig), Fc,Avitag (B7B-H82F5) is expressed from human 293 cells (HEK293). It contains AA Gly 27 - Thr 461 (Accession # Q5ZPR3-1).;This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 74.9 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Immobilized Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H3 (4Ig), Fc,Avitag (Cat. No. B7B-H82F5) with a linear range of 0.3-2 ng/mL (QC tested).;Immobilized Biotinylated Human B7-H3 (4Ig), Fc,Avitag (Cat. No. B7B-H82F5) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human B7-H3 / B7-H3 (4Ig) Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (Routinely tested).