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AdipoGen 6-JOE
Supplier: AdipoGen CDXC0687M005
Chemical. CAS 82855-40-1. Formula C23H14Cl2O9. MW 505.26. JOE is a conventional fluorophore predominantly used in automated DNA sequencing. Characterized by an intermediate-wavelength spectra, high quantum yield, and low pH sensitivity in the physiological range. Has a similar absorption and emission spectra to those of rhodamine 6G. Spectral Data lambdaex 520nm, lambdaem550 nm in Buffer pH 12 lambdaex 520nm, lambdaem 548nm in DMSO. JOE possesses an absorbance ˜520 nm and emission ˜550 nm red-shifted compared to those of carboxyfluorescein FAM. The substantial difference in emission wavelengths of FAM and JOE makes them spectrally resolvable and allows simultaneous use of both dyes in multiplex assays. JOE had been applied as a fluorescent label in DNA sequencing, PCR, and LCR amplifications.
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