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AdipoGen 7-Amino-4-trifluoromethylcouma
Supplier: AdipoGen CDXA0029G001
Chemical. CAS 53518-15-3. Formula C10H6F3NO2. MW 229.16. 7-Amino-4-trifluoromethylcoumarin AFC is used in the synthesis of substrates for the fluorometric assay of proteolytic enzymes in biological fluids and therefore the precursor for a variety of AFC enzyme substrates. It is a fluorogenic standard useful as a calibration standard for AFC-based enzyme substrates and for quantitating assays monitoring 7-amino-4-trifluoromethylcoumarin AFC release such as those utilizing ubiquitin-AFC. Compared to AMC enzyme substrates, fluorogenic AFC substrates generate AFC that has longer absorption and fluorescence wavelength that AMC. AFC is also suitable as laser dye. Spectral Data lambdaex 376nm, lambdaem 482nm, Quantum yield 0.531.
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