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Eurofins Discoverx HitHunter cAMP Assay for Biologics, 200/800 dp (96-well/384-well). (90-0075LM2)

Catalog No. NC0876723
Encompass

This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or Gαi-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Biologic drugs with a protocol optimized for use in a 96-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 2 plates (96-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.

Catalog No. NC0876723 Supplier Eurofins Discoverx Supplier No. 900075LM2
May include imposed supplier surcharges.
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This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or Gαi-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Biologic drugs with a protocol optimized for use in a 96-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 2 plates (96-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.

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