-Ketoglutarate Assay Kit is designed for biomedical research to measure -ketoglutarate levels an essential intermediate in the Krebs cycle and a key regulator of metabolic pathways This assay kit is crucial for studies involving cellular metabolism mitochondrial function and energy production It is commonly used to investigate the roles of -ketoglutarate in cancer cardiovascular diseases and neurodegenerative disorders The assay can detect activity within the nanomolar to low micromolar range facilitating precise quantitative analyses Typical applications include in vitro experiments using cell models such as fibroblasts and neurons as well as in vivo studies in animal models This kit is also beneficial in drug screening to explore the effects of potential therapeutic agents targeting metabolic pathways Experimental concentrations typically depend on the specific research design ensuring flexibility across various experimental setups