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Gibco™ AAV-MAX Helper-Free AAV Production System Kit
Description
The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production. It is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium. Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer. The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols.
Features of the AAV-MAX Helper-Free AAV Production System include:
- Components work synergistically to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
- Suspension-based system has scalable protocols from shake flask to bioreactor scale
- Simplified workflow—streamlined protocol using helper virus-free triple transfection
- Clonal, 293F-derived, high-producer cell line
- Robust transfection protocols are adaptable to large-scale transfection
- The components included in the AAV-MAX System Kit are:
- 1 vial of Viral Production Cells 2.0
- 1 L of Viral Production Medium
- 1 AAV-MAX Transfection Kit for 1 L of production
- 100 mL Viral-Plex Complexation Buffer
- 100 mL AAV-MAX Lysis Buffer
- 100 mL GlutaMAX Supplement
All components are also available for purchase separately.
Specifications
Specifications
| Cell Line | Viral Production Cells 2.0 |
| Cell Type | Kidney (Embryonic) |
| Product Type | AAV Production System |
| Sterility | Sterile |
| For Use With (Application) | AAV Production |
| Product Line | AAV-MAX |
| Quantity | Sufficient for 1 L of Culture |
| Content And Storage | • 1 vial Viral Production Cells 2.0 • 1 L Viral Production Medium • 1 AAV-MAX Transfection Kit for 1 L production • 100 mL Viral-Plex Complexation Buffer • 100 mL AAV-MAX Lysis Buffer • 100 mL GlutaMAX Supplement Storage conditions vary |
| Culture Type | Suspension Cell Culture |
For Research Use Only. Not for use in diagnostic procedures.
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