Abnova™ 15(S)-HETE ELISA Kit

Catalog No.	Quantity
89-100-164	1 Kit

Catalog No. 89-100-164 Supplier Abnova™ Supplier No. KA0307

Description

Includes

• Goat anti-Rabbit IgG Microtiter Plate
• 15(S)-HETE EIA Conjugate: A blue solution of alkaline phosphatase conjugated with 15(S)-HETE
• 15(S)-HETE EIA Antibody: A yellow solution of a rabbit polyclonal antibody to 15(S)-HETE
• Assay Buffer: Tris buffered saline containing proteins and sodium azide as preservative
• Wash Buffer Concentrate: Tris buffered saline containing detergents
• 15(S)-HETE Standard: A solution of 200,000pg/mL 15(S)-HETE
• pNpp Substrate: A solution of p-nitrophenyl phosphate in buffer. Ready to use
• Stop Solution: A solution of trisodium phosphate in water. Keep tightly capped. Caution: Caustic
• Plate Sealers

Use to measure quantitatively 15(S)-HETE in biological fluids. A competitive immunoassay.

The kit uses a polyclonal antibody to 15(S)-HETE to bind, in a competitive manner, the 15(S)-HETE in the standard or sample or an alkaline phosphatase molecule which has 15(S)-HETE covalently attached to it

Specifications

• Calibration Range: 78.1 to 20,000pg/mL
• Conjugate: AP
• Description: 15(S)-HETE ELISA Kit is a competitive immunoassay for the quantitative determination of 15(S)-HETE in biological fluids.
• Detection Limit: 69.21pg/mL
• Detection Method: Colorimetric
• Quantity: 1 Kit
• Regulatory Status: For research use only (RUO)
• Sample Type: Cell Culture Supernatant, Plasma, Serum, Urine
• Sample Volume: 100 μL
• Sensitivity: 69.21 pg/mL
• Packaging: 96-well
• Content And Storage: The standard and conjugate must be stored at -20°C; others store at 4°C. Avoid repeated freezing and thawing.
• For Use With (Application): Quantification
• Product Type: 15(S)-HETE ELISA Kit
• No. of Assays: 96