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Description
Includes
- Alpha-Fetoprotein Microplate: A 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human Alpha-Fetoprotein 1
- Sealing Tapes: pre-cut, pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay 3
- Alpha-Fetoprotein Standard: Human Alpha-Fetoprotein in a buffered protein base 80ng, lyophilized Biotinylated Alpha-Fetoprotein Antibody (50x): A 50-fold biotinylated polyclonal antibody against Alpha-Fetoprotein 170μL
- MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base 30mL
- Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant 30mL, 2 bottles
- Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate 80μL
- Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine 8mL
Specifications
Specifications
| Conjugate | HRP |
| Cross Reactivity | Bovine, Dog, Human, Monkey |
| Description | AFP (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human AFP in plasma, serum, and cell culture supernatants. |
| Detection Method | Colorimetric |
| Includes | 96-well Antibody coated microwell, 160ng Human alpha-fetoprotein standard, 1 vial Biotinylated alpha-fetoprotein Antibody (100X), 30mL mix diluent concentrate (10X), 30mL x 2 Wash Buffer 20X, 80 Streptavidin-Peroxidase Conjugate (SP Conjugate), 8mL chromogen Substrate, 12mL stop solution |
| Quantity | 1 Kit |
| Sample Type | Cell Culture Supernatant, Plasma, Serum |
| Sample Volume | 50 μL |
| Content And Storage | Store components of the kit at 4°C or -20°C as described in the protocol. |
| For Use With (Application) | Quantification |
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