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Description
Includes
- Angiotensin II Microplate (Item A): 96 wells (12 strips x 8 wells) coated with secondary antibody. 96 wells
- Wash Buffer Concentrate (20x) (Item B) 25mL
- Standard Angiotensin II Peptide (Item C) 2 vials, 10μL/vial
- Anti-Angiotensin II polyclonal antibody (Item N) 2 vials, 5μL/vial
- Assay Diluent A (Item D): contains 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. 30mL
- Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample (cell culture medium/other sample type) diluent. 15mL
- Biotinylated Angiotensin II peptide, (Item F) 2 vials, 20μL/vial
- HRP-Streptavidin concentrate (Item G): 200x concentrated HRP-conjugated Streptavidin. 600μL
- Positive control (Item M) 1 vial, 100μL
- TMB One-Step Substrate Reagent (Item H): 3, 3', 5, 5'- tetramethylbenzidine (TMB) in buffered solution. 12mL
- Stop Solution (Item I): 2M sulfuric acid. 8mL
Specifications
Specifications
| Conjugate | Biotin |
| Cross Reactivity | Human, Mouse, Rat |
| Description | AGT/Agt (Human, Mouse, Rat) ELISA Kit is an in vitro quantitative assay for detecting AGT/Agt based on the principle of Competitive Enzyme Immunoassay. |
| Detection Method | Colorimetric |
| Includes | 96-well Antibody coated microwell, 25mL Wash Buffer 20X, 2 vials Detected Polyclonal Antibody, 2 vials standard, 30mL assay diluent A, 15mL assay diluent B, 2 vials Biotinylated peptide, 8μL HRP-streptavidian concentrate, 100 |
| Quantity | 1 Kit |
| Sample Type | Cell Lysate, Culture Supernatant, Plasma, Serum |
| Sample Volume | 100 μL |
| Content And Storage | Store the kit at -20°C. For best results, please store the components as described in the protocol. Avoid repeated freezing and thawing. |
| For Use With (Application) | Quantification |
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