Learn More
Description
Includes
- Akt Microplate: 96 wells coated with anti-pan Akt antibody
- Wash Buffer Concentrate (20x)
- Assay Diluent: 15mL of 5x concentrated buffer. For diluting cell lysate sample, detection antibody
- (Item C) and HRP-conjugated anti-rabbit IgG Concentrate
- Detection Antibody Akt (Ser473): 2 vial of rabbit anti-Akt (Ser473)
- HRP-conjugated Anti-rabbit IgG, 25μL of 1,000x HRP-conjugated anti-rabbit IgG concentrate
- TMB One-Step Substrate Reagent
- Stop Solution: 2M sulfuric acid
- Cell Lysate Buffer
- Positive Control A431S003-1: 2 vial of lyophilized powder from A431 cell lysate
Specifications
Specifications
| Conjugate | HRP |
| Cross Reactivity | Human, Mouse, Rat |
| Description | AKT1 (pS473) (Human/Mouse/Rat) ELISA Kit is an enzyme-linked immunosorbent assay for the qualitative determination of human, mouse, rat phospho AKT1 (S473). |
| Detection Method | Colorimetric |
| Quantity | 1 Kit |
| Regulatory Status | For research use only (RUO) |
| Sample Type | Cell Lysate |
| Sample Volume | 100 μL |
| Packaging | 96-well |
| Content And Storage | Store the kit at -20°C |
| Show More |
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.