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Abnova™ AKT1 (pS473)/Pan AKT1 (Human/Mouse/Rat) ELISA Kit

Catalog No. 89100432 Shop All Abnova Corporation Products
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89-100-432 1 Kit
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Catalog No. 89-100-432 Supplier Abnova™ Supplier No. KA2152

Description

Includes

  • Akt Microplate: 96 wells coated with anti-pan-Akt antibody
  • Wash Buffer Concentrate (20x)
  • Assay Diluent
  • Detection Antibody Akt (Ser473): rabbit anti-phospho-Akt (Ser473)
  • Detection Antibody Akt: rabbit anti-pan-Akt
  • HRP-conjugated Anti-rabbit IgG, 25μL of 1,000x HRP-conjugated Anti-rabbit IgG concentrate
  • TMB One-Step Substrate Reagent: 12mL of 3,3',5,5'-tetramethylbenzidine (TMB) in buffered solution
  • Stop Solution: 2M sulfuric acid
  • Cell Lysate Buffer: 5mL 2x cell lysis buffer (not including protease and phosphatase inhibitors)
  • Positive Control A431S003-1: 2 vial of lyophilized powder from A431 cell lysate

Use to detect qualitatively human, mouse, rat phospho AKT1 (S473) and Pan AKT1. Enzyme-linked immunosorbent assay.

AKT1 (pS473)/Pan AKT1 (Human/Mouse/Rat) ELISA (Enzyme-Linked Immunosorbent Assay) kit is a very sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates

Specifications

Conjugate HRP
Cross Reactivity Human, Mouse, Rat
Description AKT1 (pS473)/Pan AKT1 (Human/Mouse/Rat) ELISA Kit is an enzyme-linked immunosorbent assay for the qualitative determination of human, mouse, rat phospho AKT1 (S473) and Pan AKT1.
Detection Method Colorimetric
Quantity 1 Kit
Regulatory Status For research use only (RUO)
Sample Type Cell Lysate
Sample Volume 100 μL
Packaging 96-well
Content And Storage Store the kit at -20°C
For Use With (Application) Qualitative
Product Type AKT1 (pS473)/Pan AKT1 (Human/Mouse/Rat) ELISA Kit
No. of Assays 96
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