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Description
Includes
- ZAG Microplate (Item A): 96 wells (12 strips x 8 wells) coated with secondary antibody, 96-wells
- Wash Buffer Concentrate (20x) (Item B), 25mL
- Standard ZAG Peptide (Item C), 2 vials, 10μL/vial
- Anti- ZAG polyclonal antibody (Item N), 2 vials, 5μL/vial
- Assay Diluent A (Item D): contains 0.09% sodium azide as preservative. Diluent for standards and serum or plasma samples, 30mL
- Assay Diluent B (Item E): 5x concentrated buffer, Diluent for standards and cell culture media or other sample types, 15mL
- Biotinylated ZAG peptide, (Item F), 2 vials, 20μL/vial
- HRP-Streptavidin concentrate (Item G): 100x concentrated HRP-conjugated Streptavidin, 600μL
- Positive control (Item M), 1 vial, 100μL
- TMB One-Step Substrate Reagent (Item H): 3, 3', 5, 5'- tetramethylbenzidine (TMB) in buffered solution, 12mL
- Stop Solution (Item I): 2M sulfuric acid, 8mL
Specifications
Specifications
| Calibration Range | 0.1 to 1000ng/mL |
| Conjugate | Biotin |
| Cross Reactivity | Human, Mouse, Rat |
| Description | AZGP1/Azgp1 (Human, Mouse, Rat) ELISA Kit is an in vitro quantitative assay for detecting AZGP1/Azgp1 based on the principle of Competitive Enzyme Immunoassay. |
| Detection Limit | 21pg/mL |
| Detection Method | Colorimetric |
| Includes | 96-well Antibody coated microwell, 25mL Wash Buffer 20X concentrated, detected Polyclonal Antibody 2 vials, standard 2 vials, 30mL assay diluent A, 15mL assay diluent B, Biotinylated peptide 2 vials,8μL HRP-Streptavidian concentrate, 100 |
| Quantity | 1 Kit |
| Sample Type | Cell Lysate, Culture Supernatant, Plasma, Serum |
| Sample Volume | 100 μL |
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