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Description
Includes
- EGFR Microplate: 96 wells coated with monoclonal anti-EGFR
- Wash Buffer Concentrate (20x)
- Anti-phospho-EGFR (Tyr 992): 11μL rabbit anti-EGFR (Tyr 992)
- HRP-conjugated Anti-rabbit IgG, 25μL of 500 fold concentrated HRP-conjugated Anti-rabbit IgG
- Assay Diluent: 15mL of 5x concentrated buffer. For diluting cell lysate sample, anti-EGFR (Tyr 992) and HRP-conjugated Anti-rabbit IgG
- TMB One-Step Substrate Reagent
- Stop Solution: 2M sulfuric acid
- Cell Lysate Buffer: 5mL 2x Cell Lysate Buffer (not including protease and phosphatase inhibitors)
- Positive Control A431S001-1: lyophilized powder from 100μL cell lysate
Specifications
Specifications
| Conjugate | HRP |
| Cross Reactivity | Human |
| Description | EGFR (pY992) (Human) ELISA Kit is an enzyme-linked immunosorbent assay for the qualitative determination of human phospho EGFR (Y992). |
| Detection Method | Colorimetric |
| Quantity | 1 Kit |
| Regulatory Status | For research use only (RUO) |
| Sample Type | Cell Lysate, Tissue Lysate |
| Sample Volume | 100 μL |
| Packaging | 96-well |
| Content And Storage | Store the kit at -20°C |
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