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Description
Includes
- bFGF Microplate (Item A): coated with anti-mouse bFGF, 96 wells (12 strips x 8 wells)
- Wash Buffer Concentrate (Item B): 20X concentrated solution 25mL
- Standards (Item C): recombinant mouse bFGF 2 vials
- Assay Diluent A (Item D): diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent 30mL
- Assay Diluent B (Item E): 5X concentrated buffer. For Standard/Sample (cell culture medium) diluent, 15mL
- Detection Antibody bFGF (Item F): Biotinylated anti-mouse bFGF (each vial is enough to assay half microplate), 2 vials
- HRP-Streptavidin concentrates (Item G): 300X concentrated, HRP-conjugated Streptavidin. 200μL
- TMB One-Step Substrate Reagent (Item H): of 3, 3', 5, 5'- tetramethylbenzidine (TMB) in buffered solution 12mL
- Stop Solution (Item I): 0.2M sulfuric acid 8mL
Specifications
Specifications
| Conjugate | Biotin |
| Cross Reactivity | Mouse |
| Description | Fgf2 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Fgf2. |
| Detection Method | Colorimetric |
| Includes | 96 Antibody coated microwell, 25mL Wash Buffer 20X,2 standard vials, 30mL assay diluent A, 15mL assay diluent B, 2 detection Antibody MSP vials, 8μL HRP Streptavidian concentrate, 12mL TMB one step Substrate reagent, 8mL stop solution |
| Quantity | 96 Assays |
| Sample Type | Cell Culture Supernatant, Plasma, Serum |
| Sample Volume | 100 μL |
| Content And Storage | Store the kit at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. |
| For Use With (Application) | Quantification |
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