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Abnova™ Fgf2 (Mouse) ELISA Kit

In-vitro ELISA for the quantitative measurement of Mouse Fgf2

Manufacturer:  Abnova™ KA1777

Catalog No. 89-028-512

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Includes:
  • bFGF Microplate (Item A): coated with anti-mouse bFGF, 96 wells (12 strips x 8 wells)
  • Wash Buffer Concentrate (Item B): 20X concentrated solution 25mL
  • Standards (Item C): recombinant mouse bFGF 2 vials
  • Assay Diluent A (Item D): diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent 30mL
  • Assay Diluent B (Item E): 5X concentrated buffer. For Standard/Sample (cell culture medium) diluent, 15mL
  • Detection Antibody bFGF (Item F): Biotinylated anti-mouse bFGF (each vial is enough to assay half microplate), 2 vials
  • HRP-Streptavidin concentrates (Item G): 300X concentrated, HRP-conjugated Streptavidin. 200µL
  • TMB One-Step Substrate Reagent (Item H): of 3, 3', 5, 5'- tetramethylbenzidine (TMB) in buffered solution 12mL
  • Stop Solution (Item I): 0.2M sulfuric acid 8mL

Specifications

Specifications

Biotin
Fgf2 (Mouse) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Fgf2.
Quantification
Fgf2 (Mouse) ELISA Kit
Cell Culture Supernatant, Plasma, Serum
100μL
Mouse
Colorimetric
96 Antibody coated microwell, 25mL Wash Buffer 20X,2 standard vials, 30mL assay diluent A, 15mL assay diluent B, 2 detection Antibody MSP vials, 8μL HRP Streptavidian concentrate, 12mL TMB one step Substrate reagent, 8mL stop solution
96 Assays
Store the kit at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
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